Effects and mechanisms of trophoblast cells autophagy in preeclampsia

VernacularTitle:滋养细胞自噬在子痫前期发病中的作用及其分子机制
Author: Yinyan XU ; Hui DING ; Fenfen ZHANG ; Li WANG
Publication Type:Journal Article
Keywords: Pre-eclampsia; Trophoblasts; Autophagy; Microtubule-associated proteins; Cysteine endopeptidases
From: Chinese Journal of Obstetrics and Gynecology 2013;48(10):759-762
CountryChina
Language:Chinese
Abstract: Objective To investigate the occurrence law of autophagy in trophoblast cells from preeclampsia and its underlying mechanisms.Methods Twenty cases of placenta tissues were collected from women suffered from preeclampsia and normal pregnant women respectively.Autophagosome of trophoblast cells were observed by transmission electron microscope.The expressions of LC3-Ⅱ/Ⅰ and Atg4B in placenta tissues were detected by western blot and real-time PCR.Results Compared with the control group,typical autophagosomes of trophoblast cells were observed by transmission electron microscope.The ratio of LC3-Ⅱ / Ⅰ in placenta of PE patients was increased (1.43 ± 0.23) compared with control group (0.59 ±0.12),and the expression of Atg4B was up-regulated in both mRNA [(1.73 ±0.16) folds] and protein levels (0.71 ± 0.13) compared with control group (P ＜ 0.05).Conclusions Autophagy was significantly up-regulated in trophoblast cells from patients suffered from preeclampsia.Thus,all the data suggest that autophagy might be involved in the generation of preeclampsia.