Ultrasound-mediated microbubble destruction accompanied with cationic liposome enhanced gene transfection in vitro
10.3760/cma.j.issn.1004-4477.2013.08.016
- VernacularTitle:超声微泡破裂法联合阳离子脂质体介导基因转染的实验研究
- Author:
Lu DING
;
Yunchao CHEN
;
Xiaoling LIU
;
Naxiang LIU
;
Qingping ZHANG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Cationic liposome;
Transfection
- From:
Chinese Journal of Ultrasonography
2013;22(8):691-695
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether ultrasound-mediated microbubble destruction(UMD) could enhance cationic liposome (CL) induced plasmid DNA delivery or not,and optimize the transfection conditions.Methods Multiple parameters were explored to obtain the optimal transgene efficiency by means of with or without serum in culture medium,various CL or nano-liposomal bubble(NB) concentrations,different time point of ultrasonic irradiation.The transfection efficiency was assessed by fluorescence microscopy and flow cytometer,and cell viability was evaluated by Cell Counting Kit-8 (CCK-8) assay.Results The serum could protect the cells but show little impact on transfection efficiency induced by CL.CL and plasmid DNA at a weight ratio of 4 ∶ 1 exhibited high transfection efficiency of (17.71-± 0.79)％ and high cell viability of (91.28 ± 0.76) ％.CL combining with ultrasonic irradiation at the time point of 1 hour could increase the transfection efficiency to (24.85 ± 0.78)％ (P ＜0.01).Higher transfection rate (32.47 ± 4.01) ％ was obtained by adding NB at the concentration of 10 ％ (P ＜0.05).Conclusions UMD accompanied with CL could enhance gene delivery effectively,which would provide a new method for gene therapy.
