Prediction and experimental verification of T cell reactive peptides of mycobacterium tuberculosis antigen Rv2608

VernacularTitle:结核分枝杆菌Rv2608抗原的多肽预测及实验验证
Author: Fei ZHAI ; Bingfen YANG ; Zhihong CAO ; Hongjuan AN ; Ruo WANG ; Xiaoxing CHENG
Publication Type:Journal Article
Keywords: Mycobacterium tuberculosis; Antigens; Tuberculosis/therapy; Epitopes,T-lymphocyte
From: Journal of Chinese Physician 2013;15(10):1311-1314
CountryChina
Language:Chinese
Abstract: Objective To discover T cell reactive peptides of mycobacterium tuberculosis antigen Rv2608 for treatment or adjuvant treatment for tuberculosis.Methods The T cell epitopes of Rv2608 were predicted by bioinformatics.The long-chain peptides were screened with strong affinity with HLA-A0201,HLA-A1101,and HLA-A2402.The physical and chemical characters of the peptides were predicted.The stable and hydrophilic peptides for chemical synthesis were selected.The T cell reactivity of the peptides in patients with active tuberculosis was detected by ELISPOT.The results were compared with the clinical diagnosis kits TSPOT.TB.Results Four peptides of Rv2608 were predicted and screened,and they could be active T cells of patients with active TB.Rv2608p3 was the most efficient to prime the T cell response.Conclusions Software prediction was consistent with the result of ELISPOT.Rv2608p3 might be the candidate for therapeutic vaccine for tuberculosis.