Study of the microsatellite instability involved in human gastric carcinoma and precancerous lesion
10.3760/cma.j.issn.1006-9801.2013.10.011
- VernacularTitle:胃癌及癌前病变微卫星不稳定的研究
- Author:
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Precancerous lesion;
Laser capture microdissection;
Denaturing high performance liquid chromatogragh;
Microsatellite instability
- From:
Cancer Research and Clinic
2013;25(10):689-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether microsatellite instability (MSI) is exsist in human gastric carcinoma and precancerous lesion.Methods The MSI was detected in gastric carcinoma and precancerous lesion by tissue laser capture microdissection and denaturing high performance liquid chromatogragh (DHPLC).Results MSI was found in 10.5 ％ intestinal metaplasia,21.12 ％ gastric hyperplasia and 43.75 ％gastric carcinoma respectively.In contrast,there was no microsatellite instability in normol gastric mucosa.It displayed accumulation and alteration from normal to cancerous tissues.Conclusion Pure tumor samples are obtained from tissue laser capture microdissection which could avoid the heteroplasmy of tissue.DHPLC is a high sensitive,fast and semiautomated method for identifying MSI.This experiment showed an increasing tendency of MSI incidence from precancerous lesion to gastric carcinoma.MSI could be used as a marker to predicate the occurrence of gastric carcinoma.
