Expression and significance of tumor suppressor gene Kruppel-like transcription factor 6 mRNA and protein in gastric cancer
10.3760/cma.j.issn.1006-9801.2013.10.009
- VernacularTitle:胃癌中抑癌基因Kruppel样因子6 mRNA与蛋白的表达及其意义
- Author:
Xiuwen ZHANG
;
Yinhua LIU
;
Linming LU
;
Guohai ZHAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Reverse transcriptase polymerase chain reaction;
Immunohistochemistry;
Kruppel-like transcription factors
- From:
Cancer Research and Clinic
2013;25(10):681-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between Kruppel-like transcription factor 6 (KLF6) and gastric cancer.Methods By using immunohistochemistry (IHC) and reverse transcription PCR,the expression of KLF6 protein and mRNA in human gastric carcinoma specimens and adjacent tissues from 73 patients were examined.Results The KLF6 protein expression rates were 39.7 ％ (29/73) and 90.41％(66/73) in 73 cases of gastric cancer and adjacent tissues,The KLF6 protein expression was significantly lower in human gastric carcinomas than the adjacent tissues by chi-square test (P ＜ 0.01).The KLF6 expression rates were 25.0 ％ (10/40),57.6 ％ (19/33) in poorly and well differentiated cancer.The difference between the two groups was significant (P ＜ 0.01).The optical density ratio of KLF6 mRNA were 0.1586±0.1071 and 0.8899±0.0638 in the gastric cancer and cancer adjacent tissue respectively,the difference between the two groups was significant (P ＜ 0.01).Conclusion KLF6 expression is low in gastric carcinoma,and moreover,KLF6 expression is significantly lower in poorly differentiated cancers than the well differentiated gastric cancers,there are negative correlation between KLF6 and gastric cancer,therefore KLF6 could be a useful marker for gastric cancer.
