Primary study of loss of heterozygosity on chromosome 1p36 in invasive ductal breast carcinoma

VernacularTitle:乳腺浸润性导管癌染色体1p36杂合性缺失的初步研究
Author: Ying LIN ; Xiaoyan CHEN ; Yihui HE ; Long JIN ; Xunbin YU
Publication Type:Journal Article
Keywords: Carcinoma,ductal,breast; Loss of heterozygosity; Genes,tumor suppressor; Microsatellite; 1 p36
From: Cancer Research and Clinic 2013;25(10):654-658
CountryChina
Language:Chinese
Abstract: Objective The study of loss of heterozygosity (LOH) on chromosome 1p36 was performed to locate the deletion areas probably harboring tumor suppressor genes in invasive ductal breast carcinoma not otherwise specified (IDC NOS).Methods Eighty paired breast cancer/normal tissue DNA samples were examined for LOH on chromosome lp36 using eight polymorphic microsatellite (MS) loci.The PCR products were electrophoresed on 8％ denatured polyacrylamide gel and stained using silver staining.Finally,the data were analysed and compared with the clinicopathological parameters using statistical analysis.Results In 80 IDC NOS,LOH was identified in 45 cases (56.3 ％) at least in one MS locus.MS locus D1S1310 showed the highest rate of LOH [35.7％ (25/70)].Conclusion Chromosome 1p36 might be the highly deleted region.The results of this study indicate that the chromosomal regions 1p36.23-33 might contain tumor suppressor genes associated with human breast carcinomas.