The protection of 3-AB, a PARP Inhibitor, on injures of adenohypophysis in rats with severe acute pancreatitis
10.3760/cma.j.issn.1007-631X.2013.10.018
- VernacularTitle:聚腺苷二磷酸核糖聚合酶抑制剂3-氨基苯甲酰胺对重症急性胰腺炎大鼠腺垂体损伤的保护作用
- Author:
Wenhong DENG
;
Kailiang ZHAO
;
Bo YANG
;
Qiao SHI
;
Xing ZHOU
;
Weixing WANG
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Pituitary gland;
Poly (ADP-ribose) polymerase;
3-aminobenzamide
- From:
Chinese Journal of General Surgery
2013;28(10):787-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protection of 3-aminobenzamide (3-AB),an inhibitor of Poly (ADP-ribose) polymerase (PARP),on severe acute pancreatitis associated adenohypophysis injury in rats.Method Forty Wistar rats were randomly divided into 4 groups:sham operation group (SO group,n=8),SAP group (n=12),3-AB pretreatment group (n =12),drug control group (n =8).The bilepancreatic duct was cannulated through the duodenum and SAP model was induced by a standardized pressure-controlled retrograde infusion of 5％ sodium taurocholate (0.1 ml/100 g) into the bile-pancreatic duct.In 3-AB group,3-AB (20 mg/kg) was administered via femoral vein 30 min prior to the operation;other procedures were identical to SAP group.In SO group,pancreas was flipped several times only.In drug control group,3-AB (20 mg/kg) was administered via femoral vein 30 min prior to the operation.Serum amylase,lipase were measured.Pancreas and pituitary tissue were taken for pathological examination under light microscope.PARP and NF-κB antibodies for adenohypophysis immunohistochemical stains.Adenohypophysis cell was observed under electronic microscope.Result Serum amylase,lipase and pancreas pathological scores were significantly higher in 3-AB group compared with SO group (P ＜ 0.05),but lower than that in SAP group (P ＜ 0.05).Adenohypophysis pathological injury was less severe in 3-AB group.Expressions of PARP and NF-κB in adenohypophysis cells were significantly higher in 3-AB group compared with SO group,but lower than that in SAP group (P ＜ 0.05).Ultrastructural change of thyrotroph cell was relieved in 3-AB group.No significant difference was observed between SO group and drug control group in PARP and NF-κB expression nor adenohypophysis pathological injury.Conclusions 3-AB exerts the protective effect against acute pancreatitis associated adenohypophysis injury by inhibition of PARP and NF-κB.
