Expression profile and clinical significance of microRNAs in papillary thyroid carcinoma

VernacularTitle:甲状腺乳头状癌microRNAs表达谱与临床相关性研究
Author: Chen LI ; You PENG ; Dingcun LUO ; Jinwang DING ; Wo ZHANG ; Gang PAN
Publication Type:Journal Article
Keywords: Thyroid neoplasms; Carcinoma,papillary; MicroRNAs
From: Chinese Journal of General Surgery 2013;28(9):696-700
CountryChina
Language:Chinese
Abstract: Objective To explore the expression of papillary thyroid carcinoma (PTC)-specific microRNAs in order to improve early diagnosis of PTC's and determine the invasiveness of PTC.Methods Thyroid samples from 51 cases were analyzed to investigate microRNA expression differences between benign and malignant thyroid nodules by using miRNA chip technique.The differences in the expression of microRNAs were validated by qRT-PCR,and their correlation with clinical and pathological features of PTC was analyzed.Results (1) qRT-PCR analysis showed that miR-30a-3p (U =60,P =0.003),miR-146b-5p(U =40,P =0.001) and miR-199b-5p (U =69,P =0.007) significantly different between benign and malignant tissues.(2) Expression of miR-199b-5p was significantly higher in PTC patients with margin invasion and of ipsilateral neck lymph node metastasis(P =0.010).Conclusions miR-199b-5p,miR-30a-3p and miR-146b-5p may be used to differentiate benign and malignant thyroid nodules; miR-199b-5p can help assess the invasiveness of PTC.