Effect of bone mesenchymal stem cells on lidocaine-induced apoptosis in rat dorsal root ganglion cells in vitro
10.3760/cma.j.issn.0254-1416.2013.09.013
- VernacularTitle:骨髓间充质干细胞对利多卡因致大鼠脊髓背根神经节细胞凋亡的影响:体外实验
- Author:
Xuejun SUN
;
Yan SHAO
;
Hongxing ZHANG
;
Fang ZHOU
;
Xi ZHAO
;
Hong MA
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Ganglia,spinal;
Apoptosis;
Lidocaine;
Postoperative complications
- From:
Chinese Journal of Anesthesiology
2013;33(9):1076-1078
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of bone mesenchymal stem cells (BMSCs) on lidocaine-induced apoptosis in dorsal root ganglion cells (DRGCs) of rats in vitro.Methods DRGCs in the logarithmic phase were incubated in culture plates at the density of 2 × 104 cells/cm2 (27 wells in total).DRGCs were randomly divided into 3 groups (n =9 each) using a random number table:control group (group C),lidocaine treatment group (group L) and BMSC treatment group (group B).The DRGCs in group C were incubated routinely without lidocaine,while the DRGCs were incubated for 2 h with lidocaine with the final concentration of 50 mmol/L in L and B groups.The DRGCs were then incubated normally in group L.The DRGCs were then co-cultured with the BMSCs which were incubated in Transwell chambers with the density of 2 × 104 cells/cm2 in group B.DRGCs were collected at 48 h of incubation for detection of apoptosis by flow cytometry.Apoptosis rate was calculated.Results Compared with group C,the apoptosis rate was significantly increased in L and B groups (P ＜ 0.05).The apoptosis rate was significantly lower in group B than in group L (P ＜ 0.05).Conclusion BMSCs can reduce lidocaine-induced apoptosis in DRGCs of rats in vitro,indicating that BMSCs may reduce local anesthetics-produced toxicity to the peripheral nerve.
