Multivariate analysis of the relationship between free DNA concentration in the blood and prognosis of non-small cell lung cancer
10.3760/cma.j.issn.1008-6315.2013.10.008
- VernacularTitle:血液中游离DNA浓度和非小细胞肺癌相关性多因素分析
- Author:
Haifeng GONG
;
Junfeng LIU
- Publication Type:Journal Article
- Keywords:
Non-small;
cell;
lung;
cancer;
Prognosis;
Blood;
DNA
- From:
Clinical Medicine of China
2013;29(10):1032-1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association of circulating free blood DNA and prognosis of non-small cell lung cancer (NSCLC).Methods Forty-six untreated NSCLC patients and 21 controls were selected as our subjects,and all underwent a 6 years follow-up.The level of circulating free blood DNA was determined by real-time quantitative polymerase chain reaction (qPCR) targeting the human β-globin gene.Overall survival of the patients were analyzed by Kaplan-Meier method and Cox-regression.Results At the end of follow-up,the median free DNA concentration of experimental patients was higher than that of control patients(52 μg/L vs.29 μg/L,P =0.03).The median free DNA concentration of experimental death patients (87％) died was significantly higher than that of still survived patients (59 mg/L vs.25 mg/L,P =0.02).No association of DNA concentration with tumor pathology,clinical stage,age,gender,smoking or pulmonary inflammatory conditions were found in current study.Conclusion For NSCLC patients,the circulating free blood DNA level was negative correlation with the survival of the patients,suggesting that it may be utilized as a prognostic factor for survival analysis.But there is no relationship of free blood DNA concentration with tumor pathology,age,gender,smoking or pulmonary inflammatory conditions.
