Comparison of the Chemical Composition of Subchondral Trabecular Bone of Medial Femoral Condyle between with Advanced Osteoarthritis and without Osteoarthritis.
10.11005/jbm.2015.22.3.93
- Author:
Kwang Kyoun KIM
1
;
Yougun WON
;
Tae Gyun KIM
;
Myong Hyun BAEK
;
Jaewon CHOI
Author Information
1. Department of Orthopedic Surgery, Konyang University College of Medicine, Daejeon, Korea. yougunwon@gmail.com
- Publication Type:Original Article
- Keywords:
Cartilage;
Femur;
Osteoarthritis;
Spectrum analysis raman
- MeSH:
Cartilage;
Collagen;
Femur;
Humans;
Osteoarthritis*;
Spectrum Analysis, Raman
- From:Journal of Bone Metabolism
2015;22(3):93-97
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The purpose of this study is to investigate differences of chemical composition between subchondral bone in advanced osteoarthritic (OA) and non-OA distal femur. METHODS: Twenty femurs were harvested, respectively. The subchondral trabeculae were obtained from the middle of medial articular surface of distal femurs. A 10 mm diameter cylindrical saw was used to harvest. Raman spectroscopy, a non-destructive technique, was employed to determine the chemical information of the trabecular bones in the human distal femurs. RESULTS: The maximum intensity of the phosphate peak was 2,376.51+/-954.6 for the non-OA group and 1,936.3+/-831.75 for the OA group. The maximum intensity of the phosphate peak observed between the two groups was significantly different (P=0.017). The maximum intensity of the amide I peak were 474.17+/-253.42 for the nonOA group and 261.91+/-205.61 for the OA group. The maximum intensity of the amide I peak were significantly different between the two groups (P=0.042). Also, among other chemical and matrix components (Hydroxyproline,Carbonate, Amide IIIdisordered;ordered, and CH2), the spectrums showed similar significant differences in the intensity (P=0.027, P=0.014, P=0.012; P=0.038, P=0.029). Area integration were performed to determine disorder in collagen's secondary structure via amide III (alpha helix/random coil). The value of the alpha helix to random coil band area are significantly different (P=0.021) and result showing that there was a trend toward higher collagen maturity for the nonosteoarthritic bone specimens. CONCLUSIONS: The result suggested that OA may affect the chemical compositions of trabecular bone, and such distinctive chemical information may be.
