Effects of different environmental intervention on learning-memery ability and choline acetyltransferase expression in rats after unilateral local cerebral infarction
10.3760/cma.j.issn.1674-6554.2013.09.009
- VernacularTitle:不同饲养环境对局灶性脑梗死大鼠学习记忆及胆碱乙酰转移酶的影响
- Author:
Xiangjian XIAO
- Publication Type:Journal Article
- Keywords:
Enriched environment;
Learning-memory;
Choline acetyltransferase;
MCAO
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;22(9):797-798
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different environmental intervention on the learning-memory ability and choline acetyltransferase (ChAT) expression around the infarct in rats with unilateral local cerebral infarction.Methods SD rats were subjected to electric coagulation of MCA and then were assigned randomly to ①control group(5 in each group housed in a standard cage),②learn training group(housed in a maze cage),③enriched environment group(housed in a enriched environment cage).24 days later,rats were trained by water maze,and then killed for immunohistochemistry staining.The expression of ChAT in peri-ischemic cortex was examined.Results The escape latency of rats in enriched environment was the shortest((26.9 ± 4.8)s),and the times striding over the platform was the most(6.1 ±1.1),longer (33.6 ± 5.1)s and less (4.8 ± 1.2)in the learn training group,the longest ((41.4 ± 6.4) s) and least (3.3 ± 0.9) in the control group.The positive cells of ChAT in enriched environment group (40.3 ± 8.4) were higher than those in learn training group (35.6 ± 7.8) and those in control group (25.4 ± 6.5).Conclusion Enriched environment after MCAO could promote the learning-memory ability and up-regulate the expression of ChAT in the peri-ischemic regions.
