Effect of simvastatin and atorvastatin on the expression of p 27 protein in mesangial cells of rat under high glucose
10.3969/j.issn.1671-8348.2013.28.026
- VernacularTitle:辛伐他汀与阿托伐他汀对高糖培养大鼠肾小球系膜细胞p27蛋白表达的影响
- Author:
Yanli XU
;
Qingju LI
- Publication Type:Journal Article
- Keywords:
simvastatin;
atorvastatin;
high glucose;
rat mesangial cells;
p27 protein
- From:
Chongqing Medicine
2013;(28):3400-3402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of simvastatin and atorvastatin on the level of p 27 protein in mesangial cells of rat under high glucose .Methods Cultured rat mesangial cells in vitro were divided into four groups :normal glucose(5 .6 mmol/L glu-cose) ,high glucose(30 mmol/L glucose) ,high glucose(30 mmol/L glucose) plus simvastatin(10 μmol/L) ,high glucose(30mmol/L glucose) plus atorvastatin(10 μmol/L) .After treatment for 24 ,48 ,72 120 hours ,the cells were collected .The protein level of p27 was detected by Western blotting and the mRNA level of p27 was detected by RT-PCR .Results Compared to the normal glucose group ,the levels of p27 protein and mRNA in the high glucose group had a significant increase(P<0 .05) .Compared to the high glucose group ,the level of p27 protein and mRNA in the high glucose plus simvastatin or atorvastatin group both had a significant decrease(P<0 .05) .Conclusion By modulating the expression of P27 in mesangial cells ,statins could become the independent lipid-decreasing drug to prevent kidney .
