Role of rVvhA in inducing THP-1 cells damage

VernacularTitle:重组创伤弧菌溶细胞素诱导 THP-1细胞损伤的机制研究
Author: Xiaoya LU ; Jianlin CHEN ; Biao LIU ; Danli XIE ; Yongliang LOU
Publication Type:Journal Article
Keywords: Recombinant Vibrio vulnificus cytolysin; THP-1; Cell damage; Calcium channel
From: Chinese Journal of Microbiology and Immunology 2013;(10):761-765
CountryChina
Language:Chinese
Abstract: Objective To investigate the role of recombinant Vibrio vulnificus cytolysin (rVvhA) in inducing THP-1 cells damage and study the pathway of associated calcium influx .Methods Inverted mi-croscope, CCK-8 cell proliferation kit, Fluo3/AM staining and caspase activity detection were performed to analyze the damage of THP-1 cells induced by rVvhA and the pathway of calcium influx .Results rVvhA had cytotoxic effects on THP-1 cells in a dose-dependent manner .The concentrations of extracellular K +and LDH were respectively up-regulated after 1 h and 6 h of 12 μg/ml rVvhA intervention .Verapamil , Mibe-fradil and SKF-96365 could not prevent the influx of free Ca 2+induced by rVvhA .The activities of caspase-3 and caspase-9 were singanificantly enhanced by rVvhA in a time-dependant manner .Conclusion rVvhA can induce THP-1 cells damage through triggering extracellular calcium influx via porous channel on cell membrane.Moreover, rVvhA might induce THP-1 cell apoptosis through activating caspase-9/3-dependent pathway .