Effect of silence S100A4 mRNA by RNA interference on radiosensitivity of the pancreatic cancer cell lines
10.3760/cma.j.issn.1007-8118.2013.10.015
- VernacularTitle:siRNA干扰S100A4mRNA对人胰腺癌细胞放射敏感性的影响
- Author:
Peng LI
;
Jiangwei LIU
;
Fang YUAN
;
Wenjun LI
;
Fang ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Radiation tolerance;
RNA,small interfering
- From:
Chinese Journal of Hepatobiliary Surgery
2013;19(10):777-781
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the siRNA interference of S100A4 mRNA of human pancreatic cancer cell radiosensitivity.Method Cultured human pancreatic BxPC-3,AsPC-1 in vitro,logarithmic phase cells as the experimental object,were divided into three groups:normal control group (without any treatment),negative control group (transfected with negative control fragment),interference group (transfected S100A4 protein fragment siRNA),the chemical synthesis siRNAS100A4 fragment interference of S100A4 mRNA 0,1,2,3,5,7,10 Gy given 6MV X-ray irradiation,the use of clone formation assay,Giemsa stained colony formation rate is calculated and SF2,and the fitting cell survival curve.Results The siRNA interference S100A4 after BxPC-3 cells SF2 value are:the control group 0.68±0.02,negative control group 0.65±0.01.interference group,0.38±0.02,P＜0.05.AsPC-1 cells SF2 value:control group 0.48±The0.02,negative control group 0.47±0.02; interference group:0.37±0.04,P＜0.05.Conclusion siRNA interference S100A4 after increased radiosensitivity of human pancreatic cancer cell lines.
