Protective effect of perfluorocarbon emulsions on rat donor lung
10.3760/cma.j.issn.0254-1416.2013.09.015
- VernacularTitle:乳化氟碳保存液对大鼠供肺的保护作用
- Author:
Xuemei PENG
;
Lu XI
;
Huadong WANG
;
Qingde ZHANG
;
Huihui LIU
;
Bing SHUAI
- Publication Type:Journal Article
- Keywords:
Fluorocarbons;
Emulsifying agents;
Lung transplantation;
Cytoprotection
- From:
Chinese Journal of Anesthesiology
2013;33(9):1082-1084
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the protective effect of perfluorocarbon emulsions (FCE) on donor lung of rats during storage.Methods Twenty-four healthy male Sprague-Dawley rats,weighing 350-400 g,were equally and randomly divided into 2 groups using a random number table:University of Wisconsin (UW) solution group (UW group) and FCE group (FCE group).After the model of lung perfusion was established according to the method described by Fischer et al,the lung and heart were removed and perfused with 4 ℃ UW or FCE preservation solutions.The lung was taken out when stored for 6 h for determination of SOD activity (by WST assay),malondialdehyde (MDA) content (by TBA assay),and activity of myeloperoxidase (MPO) and content of interleukin1 β (IL-1β),IL-6,tumor necrosis factor-apha (TNF-α) (using ELISA) in lung tissues and for microscopic examination of pathologic changes.Results MPO activity was significantly lower in UW group than in FCE group (P ＜0.05).There were no significant differences in the SOD activity and content of MDA,IL-1β,IL-6,TNF-α between the two groups (P ＞ 0.05).Conclusion FCE can reduce the neutrophil infiltration in lung tissues,indicating that FCE is more superior to UW solution in reduction of injury to the donor lung of rats.
