Fsp27 gene inhibits the development of CCl4-related fibrosis in rats
10.3760/cma.j.issn.1008-1372.2013.08.006
- VernacularTitle:脂肪特异性蛋白27(Fsp27)基因延缓四氯化碳所致实验大鼠肝纤维化
- Author:
Tianmin LV
;
Qiandong ZHU
;
Qiyu ZHANG
;
Fuxiang YU
- Publication Type:Journal Article
- Keywords:
Carbon tetrachloride/toxicity;
Disease models,animal;
Liver cirrhosis,experimental/pathology;
Rats,Sprague-Dawley;
Intercellular signaling peptides and proteins/genetics
- From:
Journal of Chinese Physician
2013;15(8):1028-1031
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of fat-specific protein 27 (Fsp27) gene on the regulation of liver fibrogenesis in vivo.Methods Hepatic stellate cells (HSCs) were isolated from rat liver.Fsp27 gene was detected in primary HSCs and activated HSCs by real-time quantitative PCR (RTqPCR).Lentiviral vector carrying Fsp27 gene was constructed.The model of liver fibrosis was established by infusing carbon tetrachloride (CC14).The rats with liver fibrogenesis were infected by the virus.Liver sections were made to observe the structure and form of liver histocytes.The content of fibrous protein in liver and serum was detected by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay.Resukts HSCs were isolated and cultured successfully.The difference of Fsp27 gene between primary HSCs and activated HSCs was significant(P ＜ 0.01).The model of liver fibrosis was achieved.After infecting the model rats,we found the fibrosis level in treatment group was lower compared with control group.Conclusions Fsp27 treatment can decrease collagen deposition in the liver and inhibit the formation of fibrosis.
