Regulatory effects of miR-146a on Cryptococcus neoformans-induced inflammation factors production in THP-1 cells
10.3760/cma.j.issn.0254-5101.2013.10.004
- VernacularTitle:miR-146a对新生隐球菌诱生THP-1细胞分泌炎症因子的调节作用
- Author:
Zhijun HAN
;
Hongyu HUANG
;
Min ZHANG
;
Yunyu WEI
;
Jingbo LIU
;
Zihe YAN
- Publication Type:Journal Article
- Keywords:
Cryptococcus en oformans;
miR-146 a;
Immune regulation
- From:Chinese Journal of Microbiology and Immunology
2013;(10):740-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effects of miR-146a on inflammation factors production in C ryptococcus neoformans treated THP-1 cells.Methods Cryptcoo ccus neoformans strains were heat killed .Fluorescence quantitative RTP-CR and ELISA were used to detect the levels of IL -1βand TNF-αin THP-1 cells treated with heat-killed Cryptococcus neoformans.In addition, the production of IL-1βand TNF-αwere analyzed before and after Dectin-1or TLR 4 blocked .THP-1 cell lines that were respectively transfected with miR-146 a mimics and inhibitors were constructed and the production of IL-1βand TNF-αin these cell lines were analyzed after interfered with Cryptococcus neoformans.Results With the interference of heat-killed Cryptococcus neoformans, the expression of miR-146a was up-regulated in THP-1 cells, but was down-regulated after Dectin-1 or TLR4 blocked.The expressions of IL-1βand TNF-αinduced by heat-killed Cryptococcus neoformans were enhanced in miR-146a mimics transfected THP-1 cells, but was inhibi-ted in inhibitors transfected THP-1 cells.Conclusion Heat-killed Cryptococcus neoformans could up-regu-late miR-146a expression in THP-1 cells via Dectin-1 and TLR.miR-146a could negatively regulate the ex-pressions of IL-1βand TNF-αinduced by Cryptococcus neoformans.
