Establishment of rat models of Parkinson’s disease by unilateral two-point injection with 6-hydroxydopamine

VernacularTitle:6-羟基多巴胺单侧两点注射法构建的帕金森病模型鼠
Author: Yuling WANG ; Hua GAO ; Xinling YANG
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2013;(46):8030-8035
CountryChina
Language:Chinese
Abstract: BACKGROUND:The establishment of animal models of Parkinson’s disease plays an important role in clinical and basic experiments of Parkinson’s disease, and its stability directly affected study results. OBJECTIVE:To evaluate the behavior and pathological changes of rats with Parkinson’s disease established by unilateral two-point injection with 6-hydroxydopamine.
METHODS:A total of 62 Sprague-Dawley rats were randomly assigned into experimental group (n=50) and normal group (n=12). Using stereotatic technique, 6-hydroxydopamine was injected into the right substantia nigra
RESULTS AND CONCLUSION:At 2 weeks, after induction with apomorphine, left rotation speed>7 r/min was observed in only 22 rats of the experimental group. No significant difference in the number of rotation between 2 and 4 weeks was detected (P>0.05). Tyrosine hydroxylase and dopamine content significantly reduced in the model group. Results indicated that unilateral two-point injection of 6-hydroxydopamine could be used to
successful y establish rat models of Parkinson’s disease with similar behaviors and pathologies.