In vitro biological properties of gradient strontium-containing hydroxyapatite bone cement
10.3969/j.issn.2095-4344.2013.47.002
- VernacularTitle:梯度掺锶羟基磷灰石骨水泥的体外细胞生物学性能
- Author:
Haixia WANG
;
Jin YUE
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(47):8149-8154
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Strontium-containing hydroxyapatite bone cement can improve the crystal inity and compatibility of the materials, but the effects on celltoxicity, cellsurface adhesion, proliferation and expression stil need further studies. OBJECTIVE:To observe the in vitro cytobiological properties of gradient strontium-containing hydroxyapatite bone cement.
METHODS:Four groups of strontium-containing hydroxyapatite bone cement samples were prepared at a strontium content of 0%, 1%, 5%and 10%. Six paral el samples selected from each group were subjected to ultraviolet radiation sterilization for 3 hours for standby. Dulbecco’s modified Eagle’s medium containing 10%fetal bovine serum served as extraction neurotransmitter. Strontium-containing hydroxyapatite cement powder was mixed with extract solution at a ratio of 1 g to 10 mL. L-929 fibroblasts and osteoblasts from immature rabbits were selected and identified. Scanning electron microscopy was used to observe the adhesion and proliferation of osteoblasts on the surface of gradient strontium-containing hydroxyapatite cement, and cellviability was detected using alkaline phosphatase activity method.
RESULTS AND CONCLUSION:(1) Cytotoxicity of different amount of strontium-containing hydroxyapatite cement was graded 0 or 1, suggesting the cytotoxicity of each sample was certainly associated with strontium content, reaction time, and extract concentration. (2) Hydroxyapatite bone cement containing a certain amount of strontium can promote osteoblast adhesion, proliferation and expression and improve the dissolution kinetics and biodegradability of materials, becoming more in line with the clinical requirements. But there is stil lack of long-term results.
