Influence of circadian genes hClock and hBmal1 on the chemosensitivity of gastric cancer

VernacularTitle:节律基因hClock及hBmal1对胃癌化疗敏感性的影响
Author: Junmei SONG ; Wenping ZOU ; Xin HU ; Kang LIU ; Guangming LI
Publication Type:Journal Article
Keywords: SGC-7901 Cells; circadian rhythm gene; hClock; hBmal1; chemosensitivity
From: Chinese Journal of Clinical Oncology 2013;(20):1213-1216
CountryChina
Language:Chinese
Abstract: Objective:To study the influernce of circadian genes hClock and hBmal1 on the chemosensitivity of SGC-7901 cells. Methods: SGC-7901 cells were cultivated under continuous darkness in vitro.The expression levels of the two main circadian genes hClock and hBmal1 at the different time were determined by real-time polymerase chain reaction(PCR). Docetaxel was administered at the peak and nadir time point respectively. The inhibition of SGC-7901 cell proliferation was measured using a CCK-8 kit. Result:The expression of circadian genes hClock and hBmal1 varied at different times, as shown by real-time PCR. The expression of circadian genes hClock and hBmal1 showed Phase oseillation. The maximum expression of hClock and hBmal1 mRNA was at 20:00. whereas their minimum expression was at 08:00. The inhibition ratio of docetaxel to SGC-7901 cells at the maximum expression of hClock and hBmal1 genes was lower than that at the minimum expression. Conclusion:Circadian Genes hClock and hBmal1 can reduce the drug sensitivity of SGC-7901 cell line to docetaxel in vitro.