Effect on Expression of APPmRNA in Cellular Model of AD by Therapy of Replenishing Kidney-essence and Removing Phlegm to Benefit Wisdom
10.11842/wst.2013.06.008
- VernacularTitle:补肾化痰益智法对AD细胞模型APP mRNA表达的影响
- Author:
Yuping HU
;
Ping WANG
;
Mingwang KONG
;
Depei YUAN
;
Heyuan SHI
;
Ping LIU
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
therapy of replenishing kidney-essence and removing phlegm to benefit wisdom (Bushen-Huatan-Yizhi method);
APP;
neurotoxicity
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(6):1303-1308
- CountryChina
- Language:Chinese
-
Abstract:
This study successfully established in v itro model of Alzheimerˊs disease ( AD ) in order to investigate effects and mechanisms on AD by Bushen-Huatan-Yizhi Recipe (BSHTYZR, replenishing kidney-essence and removing phlegm to benefit wisdom ) . The PC-12 cells affected by Aβ1-42 incubated were produced for neuron cell model of AD . Then , they were intervened by the serum containing BSHTYZR , which were compared to the serum containing indomethacin . The cell morphology was observed . The neuronal survival was assessed by counting MTT assay . The expression of APP mRNA was detected with RT-PCR . And the protective effect of BSHTYZR on neuronal cells and neurotoxic effects of anti-Aβ was observed . The results showed that 2 . 5 mmol/L Aβ1-42 can significantly decrease the cell viability . The RT-PCR was used on semi-quantitative analysis for expression of APPmRNA. It showed that expression of APP mRNA was increased in each group, and the BSHTYZR group can reduce the expression significantly ( P < 0 . 01 ) . It was concluded that the BSHTYZR method can protect nerve cells , suppress the expression of APP mRNA , and reduce the toxic effects .
