Enantioseparation of Salbutamol on the 3,4-Dichlorophenyl Isocyanate Vancomycin Chiral Stationary Phase
- VernacularTitle:沙丁胺醇在3，4-二氯苯基异氰酸酯万古霉素手性柱上的对映体分离
- Author:
Ping YU
;
Lei SU
;
Guang LIU
;
Li LI
- Publication Type:Journal Article
- Keywords:
Enantioseparation;
Salbutamol;
Polar mobile phase;
Chiral recognition mechanism
- From:
Journal of Kunming Medical University
2013;(10):39-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a self-made chiral column for enantiomeric separation of salbutamol. Methods We used different concentrations of acid,alkali additives in the polar phase flow for enantiomers separation of salbutamol by using 3, 4-dichlorophenyl isocyanate vancomycin chiral column, and discussed the chiral recognition mechanism. Results The ratio of acid to alkali additive in the mobile phase was 0.01%:0.01% (V/V), the flow rate was 1ml/min, the column temperature was 25℃, and the best separation of enantiomers of salbutamol was obtained,the selective factor was 1.16, the separation degree reached 1.41. Conclusion Self-made 3, 4-two chlorophenyl isocyanate vancomycin chiral column is effective for salbutamol separation, and it can be as a reference for developing other similar chiral stationary phase.
