Effect of Tangnaikang on Expression of Smad 2, 3, 7 mRNA of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β1
10.3969/j.issn.1005-5304.2013.11.014
- VernacularTitle:糖耐康对转化生长因子-β1诱导人肾小管上皮细胞Smad 2、3、7 mRNA表达的影响
- Author:
Fang LI
;
Lixia YANG
;
Chang SHU
;
Tonghua LIU
;
Lili WU
;
Wen SUN
- Publication Type:Journal Article
- Keywords:
Tangnaikang;
TGF-β1;
human renal tubular epithelial cell;
Smad 2 mRNA;
Smad 3 mRNA;
Smad 7 mRNA
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(11):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through studying the effect of Tangnaikang (TNK) on the expression of Smad 2, 3, 7 mRNA of human renal tubular epithelial cells HK-2 induced by transforming growth factor-β1 (TGF-β1), to explore the mechanism of TNK on prevention and treatment of renal fibrosis. Methods The HK-2 cells were cultured by DMEM/F12 (1∶1) with 10% fetal bovine serum and divided into control group, TGF-β1 group (TGF-β1 10 ng/mL), blank serum control group (TGF-β1 10 ng/mL + 10% animal serum), TNK drug-containing serum therapy groups (TGF-β1 10 ng/mL + 5% TNK or + 10% TNK or + 20% TNK). After 24 h, the expression of Smad 2, 3, 7 mRNA were tested by fluorescence quantitatiye PCR assay. Results After the HK-2 cells were induced by TGF-β1, the expression of Smad 2, 3 mRNA were increased and the expression of Smad 7 mRNA was decreased compared with the control group (P<0.05). The expression of Smad 2, 3 mRNA were decreased and the expression of Smad 7 mRNA was increased in TNK drug-containing serum therapy groups compared with TGF-β1 group (P<0.05), but blank serum control group had no such effect. Conclution TNK could prevent the development of renal fibrosis to some extent through regulating the expression of Smads signaling pathway.
