Content Determination of Alkaloids in Keke Tablets by HPLC
10.3969/j.issn.1005-5304.2013.11.020
- VernacularTitle:HPLC测定克咳片中生物碱成分的含量
- Author:
Yinghao ZHENG
;
Yinsheng LU
- Publication Type:Journal Article
- Keywords:
Keke Tablets;
ephedrae herba;
ephedrinehydrochloride;
pseudoephedrine hydrochlorid;
content determination;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(11):53-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for determining ephedrine hydochloride and pseudoephedrine hydrochloride in Keke Tablets by HPLC. Methods The samples were analyzed by a phemomenex Synergi Polar-RP column (4.6 mm×250 mm, 4 μm), with mobile phase methanol∶0.092%phosphoric acid (0.04%triethylamine and 0.02%second butylamine)=1.5∶98.5 at flow rate of 1.0 mL/min and detection at UV wavelength of 210 nm. The column temperature was 30 ℃. Results The linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride were 6.51×10-3-0.651 μg (r=0.999 9) and 6.27×10-3-0.627 μg (r=1), respectively. The average recoveries (n=6) of ephedrine hydrochloride and pseudoephedrine hydrochloride were 102.26%and 103.71%, with RSD of 0.34%and 0.22%, respectively. Conclusion This method is simple, accurate, reproducible, highly specific and reliable results, and has the ability to effectively control the quality of alkaloids in Keke Tablets.
