Identification of Lonicerae japonicae flos Volatile Oils by Fourier-transform Infrared Spectroscopy
10.3969/j.issn.1005-5304.2013.11.024
- VernacularTitle:傅立叶变换红外光谱法鉴别金银花挥发油
- Author:
Yan LIU
;
Tiexin TANG
;
Xinhua QIU
;
Meizhu WU
;
Xiongsi TAN
;
Kelin GAN
;
Weisheng YAO
- Publication Type:Journal Article
- Keywords:
Lonicera japonica flos;
Lonicerae flos;
volatile oils;
Fourier-transform infrared spectroscopy;
characteristic spectrum;
pattern recognition;
identification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(11):63-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a method for identification of Lonicerae japonicae flos volatile oils using Fourier-transform infrared spectroscopy. Methods The volatile oils of Lonicerae japonicae flos and Lonicerae flos was extracted by steam distillation combined with continuous liquid-liquid extraction with hexane. An oil film was prepared for Fourier-transform infrared spectroscopy scanning by dropping the volatile oils solution on the KBr disc and evaporating the solvent. The obtained infrared spectrum was treated by baseline removing and median filter smoothing. The spectral data within 1800-850 cm-1 was selected as the characteristic spectrum for hierarchical cluster analysis. And the volatile oils of Lonicerae japonicae flos and Lonicerae flos were discriminated by the result of hierarchical cluster analysis. Results Enough volatile oils were extracted for obtaining Fourier-transform infrared spectrum from small amount of Lonicerae japonicae flos. The method developed in the study was able to discriminate Lonicerae japonicae flos volatile oils from Lonicerae flos volatile oils. Conclusion The method can be used for identification of Lonicerae japonicae flos volatile oils.
