Research on the treatment and intervention of bone marrow mesenchymal stem cell to adjuvant arthritis in rats experiment
10.3969/j.issn.1671-8348.2013.29.003
- VernacularTitle:骨髓间充质干细胞对大鼠佐剂性关节炎防治的实验研究
- Publication Type:Journal Article
- Keywords:
marrow mesenchymal stem cells;
arthritis,experimental;
treatment;
intervention
- From:
Chongqing Medicine
2013;(29):3472-3475
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanisms of action about how marrow mesenchymal stem cells (MSCs) treat and inter-vene the adjuvant arthritis (AA) of rats .Methods MSCs of SD rats which had been cultured and cloned would be collected .Fre-und′s complete adjuvant was administered in rats .The rats from the model group were given MSCs treatment and intervention .Dur-ing the treatment period ,those rats′ weights ,ankle circumferences and hind paw volumes were measured .On the 21st day of the treatment ,limb-joints X-ray examination and pathological examination were taken .Results After using MSCs to treat adjuvant ar-thritis of rats ,the joint swelling of the MSCs group were relieved ,and the limb-joints X-ray showed the destructive bones had ap-parent restoration .Pathological microscopic examination of HE staining ankle joint showed joint synovial lesions significantly reduced . In the MSCs intervention group ,rats′primary inflammation and secondary lesions were not obvious ,meanwhile ,joint X-ray and pathological examination showed no obvious abnormalities .Compared with the model group ,the differences of rats′weight ,paw volume and ankle circ-umference had statistically significant (P<0 .05) .Conclusion MSCs could relieve and reverse the progression of AA .
