Correlation study on the ADAMTS-4 in early brain injury after experimental subarachnoid hemorrhage and blood-brain barrier permeability in rats

VernacularTitle:大鼠蛛网膜下腔出血后早期 ADAM TS-4的表达与血脑屏障通透性的相关性研究
Author: Sihai YUE ; Xianzhi LIU ; Hongfeng ZHANG
Publication Type:Journal Article
Keywords: ADAMTS-4; subarachnoid hemorrhage; brain injuries
From: Chongqing Medicine 2013;(29):3518-3520
CountryChina
Language:Chinese
Abstract: Objective To establish the rat model of experimental subarachnoid hemorrhage (SAH) and to detect the expression of ADAMTS-4 in early brain injury(EBI) after SAH in rats .Methods Totally 72 adult male SD rats were randomly divided into 2 groups ,the control group and SAH group .The expression of ADAMTS-4 and the content of brain Evans Blue(EB) were detected . The ADAMTS-4 inhibitor α2-macroglobulin was used to study the relationship between the expression of ADAMTS-4 and blood-brain barrier permeability .Results The expression of the mRNA and protein of ADAMTS-4 increased dramatically at 24 h and the contents of EB increased progressively at 12 h after SAH .The protein expression of ADAMTS-4 and the content of brain EB was positive correlated(r=0 .917 ,P<0 .05) .The expression of ADAMTS-4 was inhibited by α2-macroglobulin(P<0 .05) and the con-tents of EB decreased dramatically (P<0 .05) .Conclusion ADAMTS-4 may be involved in the pathological progression of early brain injury after SAH .