Effects of escitalopram on the expression of glial cell line-derived neurotrophic factor and apoptosis related factors in hippocampus of depression model rats
10.3760/cma.j.issn.1674-6554.2013.08.005
- VernacularTitle:艾司西酞普兰对抑郁模型大鼠海马胶质细胞源性神经营养因子及细胞凋亡相关因子表达的影响
- Author:
Gege YU
;
Yiming WANG
- Publication Type:Journal Article
- Keywords:
Escitalopram;
Depression;
Hippocampus;
Glial cell line-derived neurotrophic factor;
Apoptosis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;22(8):688-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanisms of escitalopram on the expression of glial cell line-derived neurotrophic factor(GDNF) in hippocampus of depression model rats.Methods 40 male SD rats were randomly divided into four groups:control group(group A),control + escitalopram group (group B),depressive group (group C),and depressive + escitalopram group (group D).Chronic mild unpredicted stress (CUMS)with solitary condition was taken to establish rat depression model.And the group B and D were treated with intragastric admistration of escitalopram(10 mg · kg-1 · d-1),the group A and C with sodium chloride.The open-field test and sucrose consumption were used to evaluate the depression behaviors of rats.The apoptotic hippocampal cells were detected by TUNEL.And the expression of GDNF,Bax,Bcl-2 and Caspase3 mRNA were detected by real-time RT-PCR.Results ① Compared with group A(12.0 ± 1.83),the number of apoptotic cells in hippocampus was significantly increased in group C (19.3 ± 1.77) (P ＜ 0.01),while group B (11.9 ± 1.91) was no significant difference (P＞ 0.05).Compared with group C,the group D(12.7 ± 1.77) had a significant reduction in the number of apoptotic cells (P ＜ 0.01).②Compared with group A,GDNF and Bcl-2 mRNA expression was decreased (P ＜ 0.01),but Bax and Caspase3 mRNA expression was both increased significantly in group C(P＜0.01) ;while in group D,GDNF and Bcl-2 mRNA expression was higher (P ＜0.01),but Bax and Caspase3 mRNA expression was lower than that in group C (P ＜0.01).Conclusion Escitalopram can improve depressive behaviors and reduce hippocampal apoptosis,which maybe associate with increasing GDNF protein and mRNA expression,and promoting the regeneration of neurons,up-regulating of mRNA Bcl-2 expression,and down-regulating of mRNA Bax and Caspase3 expression.Finally it may prevent the neuronal apoptosis in hippocampal and play the role of cerebral protection.
