Analysis of the expression of microRNA let-7e in non-small-cell lung cancer

VernacularTitle:microRNA let-7e在非小细胞肺癌中表达的研究
Author: Yiming XU ; Ping XIAO ; Chongjun ZHONG ; Liang SHEN
Publication Type:Journal Article
Keywords: Carcinoma,non-small-cell lung; microRNA; let-7e; Reverse transcriptase polymerase chain reaction
From: Cancer Research and Clinic 2013;25(9):615-618
CountryChina
Language:Chinese
Abstract: Objective To analyze the function of let-7e in the carcinogenesis of non-small-cell lung cancer.Methods The microRNA let-7e expression levels in cancer tissues and adjacent normal lung tissues were detected by quantitative real-time reverse transcription-PCR from 35 non-small-cell lung cancer patients,U6 RNA as an actin.Results The expression of microRNA let-7e in cancer tissues was significantly higher than adjacent normal lung tissues (10.111±6.135,P ＜ 0.0001),there was a significantly different between squamous carcinoma group (9.635±8.300) and adenocarcinoma group (10.301 ±5.228,P ＜ 0.05),independently of sex,smoking history,stage,and histologic characteristics of the tumor.Conclusion The expression of microRNA let-7e in cancerous tissues is high,microRNA let-7e should play oncogene role in process of non-small-cell lung cancer,and would be an useful biomarker.