Morphological Change and SHP-2 Gene Expression in Auditory Cortical Neuron after Auditory Deprivation in Rat
10.3969/j.issn.1006-7299.2013.05.020
- VernacularTitle:听觉剥夺后大鼠听皮层神经元的形态学变化和SHP-2基因的表达
- Author:
Heyin HUANG
;
Yanxing LIU
;
Ou XU
;
Yuxing WANG
;
Hong LU
- Publication Type:Journal Article
- Keywords:
Auditory deprivation;
Auditory cortex;
Morphological change;
SHP-2
- From:
Journal of Audiology and Speech Pathology
2013;(5):515-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the morphological change and SHP -2(src -homology domain contai-ning protein tygosine phosphatase type 2) gene expression in auditory cortical neuron after auditory deprivation in rat .Methods A total of 48 SD rats were randomly divided into 2-week group ,4-week group ,6-week group ,8-week group and 4 corresponding control groups with 6 rats in each group .Bilateral cochlear ablation was done to traumatized groups to ensure their postoperative ABR threshold was above 90 dB SPL .Then paraffin sectioning and HE and Nissl staining were used to detect morphological change of auditory cortical neuron ,simultaneously the RT-PCR was used to measure the expression of SHP -2 gene in auditory cortical neuron of each group ,and relative quantitative analysis was used .Results The HE and Nissl staining revealed that apoptotic shape of auditory cortical neuron became serious by time ,with the diversified cellular morphology .The relative quantity of SHP -2 gene showed statistical differences between any two groups by the method of one -way ANOVA ,showing a rising trend by time (P<0 .05) .Conclusion Along with time ,auditory cortex ,auditory deprivation showed increasing neuronic apoptosis and neuronic proliferation .Apoptosis was the final result from the mutual antagonism by the 2 factors .
