Effect of puerarin on proliferation and apoptosis of human gastric cancer MGC-803 and AGS cells

VernacularTitle:葛根素对人类胃癌细胞MGC-803和AGS增殖与凋亡的影响
Author: Xiaole MA ; Yixia YU ; Yu ZHANG ; Jinyao DONG ; Zhijie DU ; Jiansheng GUO
Publication Type:Journal Article
Keywords: Puerarin; Stomach neoplasms; Cell,MGC-803; Cell,AGS; Apoptosis
From: Cancer Research and Clinic 2013;25(9):585-587
CountryChina
Language:Chinese
Abstract: Objective To study the effects of puerarin on proliferation and apoptosis of human gastric cancer MGC-803 and AGS cells.Methods Human gastric cancer cells were treated with puerarin at different concentrations.MTT assay was used to test cell proliferation and FCM was used to detect cell apoptosis.Results The inhibition rates had upwarded trend with increasing concentrations (MGC-803:1.24 ％,2.80 ％,15.10 ％,18.55 ％,59.65 ％; AGS:15.59 ％,25.31％,30.25 ％,36.91％,64.47 ％),when treated with puerarin at different concentrations (1.5,3.0,6.0 12.0,24.0 mmol/L) for 48 hours.Apoptosis rates gradually increased with increasing concentrations (MGC-803:5.49 ％,9.53 ％,13.81％; AGS:6.23 ％,16.38 ％,25.99 ％),when treated with puerarin at different concentrations (0,12.0,24.0 mmol/L) for 24 hours.Conclusion Puerarin inhibits proliferation and induces apoptosis of human gastric cancer cells MGC-803 and AGS.