Expression and significance of PDGF-D and VEGF in gastric carcinoma
- VernacularTitle:PDGF-D和VEGF在胃癌中的表达及其意义
- Author:
Lin ZHAO
;
Caiquan ZHANG
;
Gang LIAO
;
Jiegen LONG
;
Weixue TANG
- Publication Type:Journal Article
- Keywords:
gastric carcinoma;
PDGF-D;
VEGF;
immunohistochemistry;
RT-PCR
- From:
Basic & Clinical Medicine
2009;29(12):1305-1309
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of platelet-derived growth factor-D (PDGF-D) and bascular endothelial growth factor(VEGF) in gastric carcinoma and elucidate the potential relationship between their overex-pression and clinical pathological characteristics. Methods Immunohistochemical assay was performed to detect the protein expression of PDGF-D and VEGF in tissues. Semiquantitative reverse transcription polymerase chain re-action (RT-PCR) was performed to evaluate the mRNA expression of PDGF-D and VEGF in part of random selec-ting gastric carcinoma samples. The correlation between the expression of PDGF-D and VEGF, and the relationship between the expression of PDGF-D, VEGF and the clinical pathological characteristics were analyzed. Results The protein expression of PDGF-D and VEGF in gastric carcinoma tissues were significantly higher than that in adjacent tissues and normal gastric mucosa(P <0.05) ;The expression of PDGF-D and VEGF correlated with TNM staging, depth of invasion and lymphatic metastasis (P < 0.05), while the expression of PDGF-D also correlated to histolog-ical differentiation (P < 0.05). There was a significant positive correlation between PDGF-D and VEGF at the mR-NA and protein expression levels (P < 0.05). Conclusion PDGF-D and VEGF specifically highly expressed in gastric carcinoma. The abnormal expression may play an important role in the carcinogenesis and metastasis of gas-tric carcinoma.
