Detection of serum protein biomarkers by surface enhancedlaser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) in patients with non-small cell lung cancer
- VernacularTitle:SELDI-TOF-MS技术筛选NSCLC血清肿瘤标志物
- Author:
Yunbin YE
;
Ling CHEN
;
Zhilian SHE
;
Huijing CHEN
;
Dan CAI
;
Shuoyan LIU
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
surface-enhanced laser desorption ionization time-of-flight mass spectrometry (protein fingerprinting);
carcinoma;
non-small cell lung cancer;
proteomics
- From:
Basic & Clinical Medicine
2009;29(11):1174-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristic of serum proteins in non-small cell lung cancer (NSCLC) patients, establish serum markers pattern for the diagnosis of NSCLC. Methods Surface enhanced laser desorption ionization time of flight mass spectormetry ( SELDI-TOF-MS) technology was used to analyze serum samples. Bio-marker Pattern Software (BPS) was used to detect the protein peaks. Results Sixteen significantly different pro-tein peaks were found in serum samples in NSCLC patients and healthy controls. Eight up-regulated protein peaks and eight down-regulated protein peaks ( P < 0. 001 ) were identified in serum samples of NSCLC patients. Three up-regulated protein peaks(P <0. 05) were identified in serum samples of patients of NSCLC with smoking history. Two up-regulated protein peaks(P <0. 01) were identified in serum samples of patients of squamous carcinoma comparing with adenocarcinoma. No significantly different protein peak was found in serum samples of NSCLC patients at different clinical stages . Conclusion SELDI - TOF - MS technology can identify different protein peaks and so function as a diagnostic tool with high sensitivity and specificity.
