Changes of LHR, INSR and AR genes' methylation in rat polycystic ovarian syndrome model
- VernacularTitle:多囊卵巢综合征大鼠模型LHR、INSR和AR基因甲基化的变化
- Author:
Liang ZHU
;
Fuqi XING
;
Song QUAN
;
Wenying ZHANG
;
Jianxin DU
- Publication Type:Journal Article
- Keywords:
PCOS;
DNA methylation;
animal model
- From:
Basic & Clinical Medicine
2010;30(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of PCOS and to detect the DNA methylation states of LHR, INSR and AR genes. Methods 24-days-old female Sprague-Dawley (SD) rats were randomly divided into two groups. The rats in the experimental group were given subcutaneous implanting of levonorgestrel silica gel staff and injected 1.5 IU hCG twice daily for 9 days from the 4th day. The rats in the control group were injected with normal saline at the same time. Ovarian morphologic changes, sex hormone levels, fasting serum insulin and glucose were detected. The LHR, INSR and AR genes' DNA methylation patterns were checked by methylation specific PCR in modeling group and control group. Results The ovarian weight and volume in modeling group were higher than those in control group. The ovaries in modeling group showed multiple follicular cysts, and the number of theca cells and interstitial cells increased. Less developed follicles and corpus lutea were seem. The serum level of progesterone, testosterone, luteinizing hormone, fasting insulin and glucose were significantly higher in experimental group than those in control group, so as the LH/FSH ratio and HOMA index. No methylation of LHR and AR genes were found in both groups. The methylation frequency of INSR gene (76.7%) was significantly higher in modeling group than that in control group (P<0. 001). Conclusion The depression of INSR gene's transcriptional induced by DNA methylation is important in the development of insulin resistance in PCOS.
