Increased phosphorylation of CaMK Ⅱ in cerebral tissues from hypoxially preconditioned mice
- VernacularTitle:低氧预适应小鼠脑组织内CaMKⅡ磷酸化水平增高
- Author:
Haitao LI
;
Jun JIANG
;
Weiwei YANG
;
Xiangning BU
;
Song HAN
;
Junfa LI
- Publication Type:Journal Article
- Keywords:
HPC;
CaMKⅡ;
hippocampus;
cortex
- From:
Basic & Clinical Medicine
2010;30(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of calcium/calmodulin-dependent protein kinase Ⅱ ( CaMK Ⅱ ) in the development of cerebral hypoxic preconditioning(HPC). Methods Healthy male BALB/c mice were randomly divided into 7 groups as follows; normoxic control (H0) , early(H1～H4) and delayed (H5～H6) hypoxically preconditioned mice groups. SDS-PAGE, Western blot and Gel Doc imagine systems were applied to quantitatively analyze the level of CaMK Ⅱ phosphorylation and protein expression level in the brain of mice. Results Compared with H0 group, the phosphorylation level of CaMK Ⅱ increased in cortex and hippocampus of mice in H3～H5 hypoxically preconditioned groups (P<0.05). However, there was no significant changes in total CaMK Ⅱ protein expression in cortex and hippocampus of hypoxic preconditioned mice. Similarly, enhanced p-Thr286 CaMK Ⅱ was also observed in the hippocampus and cortex of mice by immunostaining following hypoxic exposures (H3 and H6). Conclusion The increased phosphorylation of CaMKⅡ may be involved in the development of cerebral HPC in mice.
