Determination of Sulfadiazine Residues in Pork by Molecular Imprinted Column Coupling with High Performance Liquid Chromatography
10.1016/S1872-2040(11)60558-6
- VernacularTitle:分子印迹柱-高效液相色谱法测定猪肉中磺胺嘧啶
- Author:
Yunhong HUANG
;
Yang XU
;
Bibai DU
;
Qinghua HE
;
Yusheng CAO
- Publication Type:Journal Article
- Keywords:
Molecular imprinted column;
High Performance Liquid Chromatography;
Sulfadiazine;
Pork
- From:
Chinese Journal of Analytical Chemistry
2012;40(7):1011-1018
- CountryChina
- Language:Chinese
-
Abstract:
A method for the quantitative monitoring of sulfadiazine (SD) residues in pork was established by molecular imprinted column coupling with high performance liquid chromatography (HPLC).The molecular imprinted column was selected as an extraction device.To obtain the optimum extraction efficiency,several parameters related to the molecular imprinted column,including column solvent,flow-rate,eluent of the sample matrix and eluent volume,were investigated.The sample solution was directly injected into the device for the extraction after simple extraction.Under the optimum conditions,the relative standard deviations (RSD) was ≤6.1％ and the recoveries for SD were higher than 75.6％.In comparison with the AL-SPE column,the MIP-SPE column had good reusability and extraction efficiency.This method was successfully applied to the determination snlfadiazine residues in pork.
