ASPS induces G_2/M arrest of H446 cells by activation of ERK signal pathway
- VernacularTitle:刺五加多糖通过ERK信号转导途径诱导H446细胞G_2/M期阻滞
- Author:
Junxia ZHAO
;
Yongxin YAN
;
Yanling WANG
;
Shuo HAN
;
Yunli YAN
- Publication Type:Journal Article
- Keywords:
acaruhopanax senticosus polysaccharide;
small cell lung cancer cell lines (H446);
G_2/M arrest;
ERK
- From:
Basic & Clinical Medicine
2010;30(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate ASPS induced G_2/M arrest in lung cancer cell line H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis was used to inspect the proteins of ERK, p-ERK. Results Compared with control group, G_2/M phase cells increased with concentration significantly, G_0/G_1 phase cells were not different, G_2/M phase cells and G_0/G_1 phase cells were not different when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, protein of p-ERK was significantly increased, expression of ERK was no different. Conclusion ASPS may induce G_2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.
