Ligustrazine inhibited TNF-α-induced TGF-β1 and CTGF expression in human peritoneal mesothelial cells
- VernacularTitle:川芎嗪抑制TNF-α诱导的人腹膜间皮细胞TGF-β1和CTGF的表达
- Author:
Guisong ZHU
;
Jinsong HE
- Publication Type:Journal Article
- Keywords:
Ligustrazine;
tumor necrosis factor-a;
human peritoneal mesothelial cells;
transforming growth factor-β1;
connective tissue growth factor
- From:
Basic & Clinical Medicine
2009;29(11):1185-1188
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Ligustrazine on TNF-α-induced TGF-β and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin di-gestion. Then, the subcultured HPMCs were divided into control group, TNF-α-induced (1 μg/L) group and TNF-α-induced plus low-, medium-and high-dose Ligustrazine (10, 20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-β1 and CTGF mRNAs in HPMCs. TGF-β1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results. Results Ligustrazine significantly decreases TNF-α-induced TGF-β1 and CTGF expression in a dose-dependent manner at both protein and gene levels ( P < 0. 05 ). In addition, medium-and high-dose Ligustrazine injection significantly ameliorates the viability of HPMCs inhibited by TNF-α ( P < 0. 05 ). Conclusion Ligustrazine inhibits expressions of TGF-β and CTGF of HPMCs in an inflammatory conditions.
