Effect of phosphorylated-P38 MAPK on caspase-3 expression in substantia nigra of the MPTP mouse model of Parkinson's disease
- VernacularTitle:磷酸化P38 MAPK对帕金森病MPTP模型小鼠黑质caspase-3表达的影响
- Author:
Zifeng WEI
;
Yongsheng WANG
;
Qian WANG
;
Liren MA
;
Zuofeng ZHANG
;
Junling GAO
;
Yuxin ZHANG
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
caspase-3;
p-P38 MAPK;
TH;
MPTP
- From:
Basic & Clinical Medicine
2010;30(1):54-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investegate the effect of phosphorylated-P38 MAPK(mitogen-activated protein kinase) on the expression of caspase-3 in the substania nigra (SN) of MPTP-induced mouse model of(PD). Methods Mice were randomly divided into MPTP model group, which were treated with MPTP and inhibitor group. Once a day for 5 days; control group was treated with saline and DMSO as much as the model group received per day for 5 days. The behavioral were observed, immunohistochemistry and Western blot for TH, caspase-3 and phosphorylation of P38 MAPK were used to observe the change of positive cell number and the expression level in the SN of midbrain. Results Compared with the mice in control group, the model group showed typical symtoms of PD with decreased numbers of TH-positive neurons and the protein level of TH in SN of the midbrain by about 60% and 65% respec-tively(P<0.01) , the numbers of caspase-3 and phosphorylation of P38 MAPK immunoreactive cells and their protein level in the SN of the midbrain increased markedly (P<0.01). After giving SB203580, the above changes were reduced obviously (P <0. 01). Conclusion In the mouse model of subacute Parkinson's disease induced by MPTP, phosphorylated-P38 MAPK regulated caspase-3 in the SN of midbrain, the specific P38 MAPK inhibitor SB203580 is neurologically oprotective to the mouse model.
