Transplantation of allogenic mesenchymal stem cells up-regulates connexin 43 expression in rats with myocardial infarction
- VernacularTitle:同种异体骨髓间充质干细胞移植上调急性心肌梗死大鼠Cx43的表达
- Author:
Jinyi LI
;
Guoqiang ZHONG
;
Honghong KE
;
Yan HE
;
Lina WEN
;
Zhuo WEI
;
Yanmei ZHAO
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
myocardial infarction;
Connexin 43
- From:
Basic & Clinical Medicine
2010;30(4):337-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the alterations of connexin 43 (Cx43) expression and its distribution at different stages of myocardial infarction (MI) in rats after transplantation of allogenic mesenchymal stem cells (MSCs).Methods Wistar rats were ligated on the left anterior descending coronary artery to make MI models.They were injected with allogenic MSCs,which were induced by 5-aza and labelled by DAPI,during the second operation after 7 days of MI.In subgroups,MSCs were detected by fluorescence microscope.Cx43 expression and GJ distribu-tion were examined by immunohistochemistry after 4,8 or 12 weeks respectively.Results MSCs differentiated into cardiac muscle cell-like cells which were capable of pulsing spontaneously,expressing cTnT and forming myofilament in vitro.Transplanted MSCs can survive in MI host and upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.No change of Cx43 was seen at infarcted zones.Conclusion MSCs have the plasticity of differentiating into cardiac muscle cell-like cells which can continuously upregulate Cx43 expression and normalize Cx43 distribution at ischemic zones after 4,8 and 12w.
