Effect of all-trans retinoic acid on expression ofα-SMA in rat model with pulmonary hypertension
10.3969/j.issn.1000-3606.2013.11.018
- VernacularTitle:全反式维甲酸对肺动脉高压大鼠肺组织中α-SMA表达的影响
- Author:
Hong XIANG
;
Wenshen ZHAO
;
Jinquan LU
- Publication Type:Journal Article
- Keywords:
pulmonary arterial hypertension;
monocrotaline;
all-trans retinoic acid;
α-smooth muscle actin
- From:
Journal of Clinical Pediatrics
2013;(11):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To explore the possible mechanism of all-trans-retinoic acid (ATRA) therapy for pulmonary ar-terial hypertension by observing the effect of ATRA on the expression ofα-smooth muscle actin (α-SMA) in monocrotaline (MCT)-induced rats with pulmonary hypertension. Methods Thirty SD rats were randomly divided into control group, MCT-induced pulmonary hypertension group (model group) and ATRA treatment group (therapeutic group). MCT (60mg/kg) was injected to the rats in the model group and the therapeutic group to induce pulmonary arterial hypertension. And at the same day ATRA [30mg/(kg?d)] was given to rats in the therapeutic group and continually given for 28 days. The mean pulmonary arte-rial pressure and right ventricular hypertrophy index (RVHI), percentage of wall thickness (WT%) and percentage of wall area (WA%) of pulmonary arterioles were evaluated at day 28. The expression ofα-SMA mRNA and protein in the lung tissue were detected by Real-time PCR and Western blot. Results Mean pulmonary arterial pressure, RVHI, WT%and WA%were signiif-cantly higher in the model group than those in the therapeutic group (P<0.01) but these data in the therapeutic group were higher than that in the control group (P<0.01). The expression ofα-SMA mRNA and protein were higher in the model group than those in the control group and the therapeutic group (P<0.01), while there was no difference was found between the therapeutic group and the control group (P>0.05). Conclusions ATRA has therapeutic effect on pulmonary hypertension through down-regulation ofα-SMA expression.
