Secretion of adipokines in miR-26b over-expressed human adipocytes differentiation process

VernacularTitle:miR-26b过表达对不同时间点人脂肪细胞分泌脂因子的影响
Author: Chunmei SHI ; Guangfeng XU ; Chenbo JI ; Ling CHEN ; Lei YANG ; Lingxia PANG ; Yaping ZHAO ; Xirong GUO
Publication Type:Journal Article
Keywords: miR-26b; adipokines; obesity; human adipocytes
From: Journal of Clinical Pediatrics 2013;(10):914-916
CountryChina
Language:Chinese
Abstract: Objective To investigate the inlfuence of overexpression of miR-26b on the secretion of adipokines dur-ing human adipocyte differentiation. Methods Human preadipocytes were infected with the hsa-miR-26b over-expressing lentivirus and were induced to differentiate, and then the levels of adipokines (IL-6, leptin, resistin, TNF-α) at different time points during differentiation were measured by ELISA. Results Compared with control group, decreased secretions of both IL-6 and leptin, and increased secretion of resistin were found during the differentiation of human adipocytes in miR-26b overexpressed group. However, the secretion of TNF-αwas not measured in both groups. Conclusion The miR-26b can improve the inlfammation and insulin resistance of human adipocytes, which will provide potential targets for obesity treat-ment.