Early diabetic nephropathy plasma proteomic analysis
10.3969/j.issn.1671-8348.2013.32.006
- VernacularTitle:早期糖尿病肾病的血浆蛋白质组学分析
- Author:
Hongmei LU
;
Shuifu TANG
;
Kunhua HU
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
proteomics;
2-D DIGE
- From:
Chongqing Medicine
2013;(32):3867-3869
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the sensitive blood plasma molecular markers in diabetic nephropathy (DN) .Methods Two-di-mensional fluorescence difference gel electrophoresis (2D-DIGE) was used to analyze early DN patients plasma (n=8) and normal plasma ,proteins that showed differential expression of a 1 .5 fold change were analyzed by MALDI-TOF/TOF mass spectrometry . Results 2D-DIGE maps of plasma proteins in patients with DN and normal plasma were established successfully .We validated 13 differentially expressed proteins detected by 2D-DIGE ,including complement component C3、complement component C4、apolipopro-tein E ,etc .Conclusion Proteomic analysis can objectively revealed the differences of protein expression between the two kinds of blood plasma .The 13 proteins might be the potential plasma molecular markers for the clinical diagnosis of diabetic nephropathy .
