Study of the level and function of Th17 cells in the peripheral blood of asthmatic

VernacularTitle:哮喘患者外周血Th17细胞水平及功能的研究
Author: Zeyu LIU ; Shuyue XIA ; Ran WANG
Publication Type:Journal Article
Keywords: asthma; interleukin-17; Th17 cell; RORγt
From: Chongqing Medicine 2013;(31):3766-3768
CountryChina
Language:Chinese
Abstract: Objective To explore the role of Th17 cells in the pathogenesis of asthma .Methods Peripheral blood was obtained from 10 patients with asthma(asthma group) and 10 healthy volunteers(control group) .RORγt mRNA expression of lymphocytes was measured by RT-PCR ,percentage of Th17 cell was detected by FCM ,IL-17 in plasma was examined by ELISA .Results RORγt mRNA level in asthma group(0 .46 ± 0 .07) was significantly higher than that in control group (0 .15 ± 0 .02) ,proportion of Th17 cell in asthma group(28 .53 ± 7 .20)% was significantly higher than that in control group(14 .72 ± 2 .33)% ,P<0 .01 .Com-pared with IL-17 level in control group(59 .68 ± 8 .85)pg/mL ,there was significant increase in asthma group(102 .31 ± 11 .45)pg/mL(P<0 .01) .Conclusion Th17 cell is associated with asthma ,and probably aggravating asthma condition through increasing in-flammation secreting IL-17 .