The experimental study of the inhibitory effect of human recombinant sTRAIL targeting activated by u-PA on the colon cancer

VernacularTitle:以u-PA 为靶向分子的重组人 TRAIL 特异激活抗结肠癌的实验研究
Author: Dan MA ; Chaojun ZHANG ; Zulin CHEN ; Hua YANG
Publication Type:Journal Article
Keywords: arinary plasminogen activator; apoptosis; colon neoplasms; TNF related apoptosis inducing ligand
From: Chongqing Medicine 2013;(33):3992-3994
CountryChina
Language:Chinese
Abstract: Objective To make better use of the antitumor effects of TNF related apopotosis inducing ligand (TRAIL) ,we con-struct a new fusion protein including TRAIL ,for the sake of improving its purity and targeting to hepatocytes .Methods Fusion protein concluding His label protein and human recombinant TRAIL which linked by constructed specifically u-PA cleavage site . TRAIL protein was obtained by disintigrating from the fusion protein which had been purified .Results The purified His-rh-sTRAIL was demonstrated to be cleavable by u-PA and possess enhanced antitumor effect ,it′s toxicity to hepatocytes was reduced . Conclusion The fusion protein His-rhsTRAIL has antitumor effect on colon cancer cell with high efficiency ,highly targeting and low-toxicity .