Establishment and evaluation of model for adult rats with vitamin D deficient
10.3969/j.issn.1671-8348.2013.32.029
- VernacularTitle:成年维生素D缺乏大鼠模型的建立及评价
- Author:
Qinghua LI
;
Fengxiang ZHANG
;
Lijun SHAO
;
Fei WANG
;
Qiang ZHAI
;
Bo LIAN
;
Weiwei YANG
;
Guohui WANG
- Publication Type:Journal Article
- Keywords:
vitamin D deficiency;
SD rats;
hypertension
- From:
Chongqing Medicine
2013;(32):3923-3925
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate animal model of adult rats with vitamin D deficient and assess the association of vitamin D deficiency and hypertension .Methods Adult SD rats were divided into 3 groups :normal controls group(feed with ordina-ry forage) ,vitamin D deficient groups(feed without vitamin D)and vitamin D control group(feed with vitamin D) ,which were fed with different diets under 12 h:12 h without ultraviolet light bulb(UV-B) .After treatment for 6 weeks ,Retinal blood was collect-ed ,and Serum vitamin D levels was tested by EIA every 2 weeks .Results After treatment for 10 weeks ,Serum level of vitamin D in vitamin D deficient group were reduced to 2 .07 nmol/L ,while vitamin D group and normal control group were measured as 56 .74 nmol/L and 57 .61 nmol/L ,there was no statistically significant difference between vitamin D group and normal control group (P>0 .05) .hypertension of vitamin D deficient group was significantly higher than other two groups .Conclusion It is feasible to estab-lish model of adult rats with vitamin D by UV-B free light and diets control ,hypertension of rats vitamin D deficiency has a signifi-cant rise compared with the control group .
