Rhein on diabetic nephropathy rat podocytes nephrin expression
10.3969/j.issn.1671-8348.2013.31.004
- VernacularTitle:大黄酸对糖尿病肾病大鼠足突细胞nephrin基因表达的影响
- Author:
Jianwei CHEN
;
Ling LIU
;
Ling ZHONG
- Publication Type:Journal Article
- Keywords:
rhein;
diabetic nephropathy;
nephrin
- From:
Chongqing Medicine
2013;(31):3732-3734
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate rhein on podocyte nephrin gene expression and nephrin on glomerular in diabetic nephropa-thy (DN)rats .Methods DN rats mode were made by Intraperitoneal injection of streptozotocin (STZ) 60 mg/kg once .the DN model was divided into 2 groups:rhein treatment group (A) ,model group (B) ,and in addition ,set up a control group (C) .body weight ,kidney weight ,relative kidney weight ,24-hour urinary protein excretion ,serum creatinine ,blood urea nitrogen of rats were detected after 8 weeks of intervention .kidney pathological changes were observed by using light microscope ,electron microscope . immunohistochemical technique used to nephrin protein expression .Results Both the DN rats 24 -hour urinary protein excretion and podocyte nephrin gene expression of group A decreased significantly (P<0 .05) ,improvement in renal function .Conclusion Rhein on DN kidney has a protective effect ,which may be related to its podocyte nephrin gene expression.
