Human placenta-derived mesenchymal stem cell transplantation promotes tendon graft healing in a bone tunnel
10.3969/j.issn.2095-4344.2013.49.012
- VernacularTitle:人胎盘来源间充质干细胞移植促进肌腱移植物在骨隧道内的愈合
- Author:
Lifeng YANG
;
Wei LIU
;
Ye ZHOU
;
Zishan FENG
;
Li ZHANG
;
Wei ZHAO
;
Zhi LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(49):8539-8544
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that human placenta-derived mesenchymal stem cells with strong proliferative ability have rich sources and can remarkably promote tendon-bone healing after celltransplantation.
OBJECTIVE:To observe the effect of human placenta-derived mesenchymal stem cells on tendon-bone healing in a bone tunnel.
METHODS:Human placenta-derived mesenchymal stem cells were separated using adherent separation screening method. Thirty 8-week-old male Sprague-Dawley rats were randomly divided into two groups, 15 rats as experimental group and 15 rats as control group. Experimental group were subjected to transplantation of human placenta-derived mesenchymal stem cells and the control group were injected with saline solution.
RESULTS AND CONCLUSION:Stem cells, new vessels, and fibrocartilage hyperplasia were observed on the tendon-bone interface with microscope at 2, 4 and 6 weeks after celltransplantation in the experimental group. Biomechanical y, the maximum pul out load in the experimental group was significantly higher than that in the control group at 4 and 6 weeks after celltransplantation (P<0.05). These findings suggest that human placenta-derived mesenchymal stem cells can accelerate early tendon-bone healing in a bone tunnel and strengthen the biomechanical strength.
