The Diagnostic Effect of Brainstem Evoked Potential for Pancreatic Encephalopathy in Rats with Severe Acute Pancreatitis
- VernacularTitle:脑干诱发电位对重症急性胰腺炎大鼠脑损伤的诊断作用
- Author:
Bo PENG
;
Zheng WU
;
Huanchen SHA
;
Qingyong MA
;
Xianhua LUO
;
Cui ZHANG
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
Brainstem evoked potential;
Pancreatic encephalopathy
- From:
Journal of Kunming Medical University
2013;(11):9-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is to demonstrate the diagnostic effect of brainstem evoked potential for pancreatic encephalopathy in rats with severe acute pancreatitis. Methods Sixty male Sprague-Dawley (SD) rats were randomly divided into two equal groups: a sham-operated (SO) group and a severe acute pancreatitis (SAP) group. Each group was evaluated at 3, 6, and 12 h during the experiment. To detect the brain stem evoked potential change at different time points. The ultrastructure of brain tissue was observed by transmission electron microscope (TEM). The expressions of the apoptosis-related proteins Bcl-2, Bax and caspase-3 were observed using immunohistochemical and Western Blot technique. Results In SAP group, congestion, edema, inflammatory cell infiltration, mitochondrial swelling and cell apoptosis were apparent. Compared with SO group, the brain stem evoked potential in severe acute pancreatitis group was obviously reduced in SAP group. Compared with SAP group, the expressions of Bcl-2 have increased, whereas the expressions of Bax and caspase-3 have decreased in SO group significantly ( <0.05) . Conclusion Brain stem evoked potential is a sensitive method in detection of rat brain damage. The results showed that the consistency and the damage degree of rats may be important clinical diagnostic index of pancreatic encephalopathy.
